CCQM meeting of the working groups CAWG, NAWG, and PAWG

7 Oct 2024, 08:00 → 11 Oct 2024, 14:00 Europe/Berlin

PTB Berlin

Claudia Swart (Physikalisch-Technische Bundesanstalt)

This meeting includes the autumn meeting of the individual CCQM working groups CAWG, NAWG, and PAWG, followed by a joint meeting of all three working groups as well as the stakeholder workshop "Towards standardisation of pathogen measurements".

Monday 7 October

08:00 Registration on site

Please get your name tags and meeeting documents prior to the start of the meeting.

NAWG meeting: Session 1

1 Introductions (‘tour de table’ for those present, with online participants providing name and institution in the chat box). Agree agenda Agree minutes NAWG18– review action list

Speakers: Jim Huggett, all

2 General update on recent activities: K176, Good news stories

Speakers: Jim Huggett (NML@LGC), all

PAWG meeting: 1st Session - Miniworkshop

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

3 Welcome and introductions of participants

Speaker: Claudia Swart (Physikalisch-Technische Bundesanstalt)

4 IDMS for proteins and peptides: historical development and approaches

Speaker: G. O'Connor (PTB)

5 Insights from the use of solution calibrants from three metrology institutes for the measurement of urine albumin via IDMS

Speaker: H. Tan (HSA)

6 Use of protein internal standard and its influence on the overall uncertainty

Speaker: A. Green (NIST)

10:30 Coffee Break

NAWG meeting: Session 2

7 CCQM-K189 INDEL/SNV

Update on study progress

Speaker: NIM

8 CCQM-K181/P227 SARS-CoV-2 RNA copy number quantification

Update on Draft B. KCRV, DOE and CMC claims

Speaker: NIM

PAWG meeting: 2nd Session - Miniworkshop

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

9 Quantification of troponin using protein or peptide-based calibrators

Speaker: M. Öztug (TÜBITAK)

10 Calibration of RMPs with peptides Vs recombinant proteins: the example of procalcitonin and pTau

Speakers: A. Boeuf (LNE), C. Giangrande (LNE)

11 Quantification of tHb in matrix with peptide and protein as calibrators: experience from CCQM P201

Speaker: Y. Liu (NIM)

12 Discussion and recommendations

12:30 Lunch Break

CAWG meeting: Session 1

13 Welcome and register, Confirm/Appoint Rapporteur, Agree agenda, Review minutes and actions, Spring 2024 meeting

Speaker: Julian Braybrook (NML@LGC)

14 P205 “Enumeration of membrane intact E.coli”

Speaker: Boqiang Fu (NIM)

15 P197 “Proliferative stem cell number per unit area”

Speaker: Nilofar Faruqui (NPL)

16 P222 “Particle enumeration for cellular analysis”

Speaker: Yuki Kuruma (NMIJ)

NAWG meeting: Session 3

17 Orthogonal study

Proposed study

Speaker: NMIA

18 SARS KC

Update on proposed study

Speaker: NML@LGC

19 RNA Lipid Nanoparticles (LNPs)

Update on proposed study

Speaker: NIST

PAWG meeting: 3rd Session - Comparisons

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

20 Follow-up investigations HbA1c

Speaker: Q. Liu (HSA)

21 CMCs based on CCQM-K177

Speaker: C.-B. Arsene (PTB)

22 Update on CCQM K186/P238

Speaker: C. Brauckmann (PTB)

23 Update on CCQM K115d/P55.2d

Speaker: G. Martos (BIPM)

24 Fire drill exercise

Speakers: G. O'Connor (PTB), J. Melanson (NRC)

15:30 Coffee Break

CAWG meeting: Session 2

25 P217 “Quantification of fixed mononuclear cells in suspension”

Speakers: Deepa Rajagopal (NIST), Sumona Sarkar (NIST)

26 Assessment of current work programme; new Pilot studies, Key Comparison studies. Discussion

Speaker: all

NAWG meeting: Session 4

27 CCQM NAWG Bio CMCs /Review

Update on BioCMC review process
Review BioCMC 2024-25
cycle nominations process

28 Food task group

Update and questionnaire

Speaker: INM

PAWG meeting: 4th Session – Task groups

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

29 Update task group on food

online

Speaker: C. Portesi (INRIM)

30 Task group I

Speaker: R. Josephs (BIPM)

31 Task group II

Speaker: C.-B. Arsene (PTB)

32 Task group III

Speakers: L. Wu (NIM), M. Ryadnov (NPL)

33 Closing for the day

Speaker: Claudia Swart (PTB)

Welcome Reception

Fingerfood and Drinks

Tuesday 8 October

CAWG meeting: Session 3

34 1) Review of CAWG Strategic plan and National needs identified at last meeting; identification of cell metrology opportunities

Speaker: all

NAWG meeting: Session 5

35 CCQM-P231: Species specific meat composition determination of DNA extracted from meat

Overview of Pilot background, protocol samples & aims
Results summary & draft report/publication discussion
– Material 1
– Material 2
Update on study
5 minute participant presentation of results & MU calculations (reporting slides can be found here https://bit.ly/3ArlmOF )

Speaker: TÜBITAK

PAWG meeting: 1st Session – scientific presentations

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

36 Discovery of pharmabiotics from human gut microbiome

Speaker: D. Kang (KRISS)

37 Absolute quantification of active protein by SPR and digital ELISA

Speaker: L. Wu (NIM)

38 Structure related measurements

Speaker: M. Ryadnov (NPL)

10:30 Coffee Break

CAWG meeting: Session 4

39 2) Review of CAWG Strategic plan and National needs identified at last meeting; prioritization of cell metrology opportunities

Speaker: all

NAWG meeting: Session 6

40 CCQM-P231: continued

5 minute participant presentation of results & MU calculations (reporting slides can be found here https://bit.ly/3ArlmOF)

Speaker: TÜBITAK

41 CCQM-K86.d DNA Ratio in a High Protein Matrix

Update on Draft. KCRV, DOE and CMC claims

Speakers: NIMT, TÜBITAK

42 P232 Fire drill

Update on study report

Speakers: NIST, NML@LGC

PAWG meeting: 2nd Session - scientific presentations and digitalisation

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

43 Characterization and Quantification of Monoclonal Antibodies by IDMS and ES-DMA-CPC

Speaker: W. Mi (NIM)

44 Use of the digital expert

Speaker: D. Hutzschenreuther (PTB)

45 Digitalization experience in protein measurement in NIM

Speaker: L. Wu (NIM)

12:30 Lunch Break

CAWG meeting: Session 5

46 3) Agreement on Next steps; Wrap-up

Speaker: all

NAWG meeting: Session 7

47 Potential topics/Planned activities KCs/pilots

Planned studies measuring bacterial nucleic acids
ctDNA SNV KC
eDNA
SOGAT, sequencing activities
ISO/CLSI update

Speakers: KRISS, NIB

PAWG meeting: 3rd Session - strategy

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

48 Strategy

Speakers: Claudia Swart (PTB), all

49 CMC Guidelines

Speakers: Claudia Swart (PTB), all

50 Uncertainty evaluation issues

Speakers: L. Wu (NIM), all

15:00 Coffee Break

NAWG meeting: Session 8

51 Wrap up

Review GANTT chart chart timelines
Strategy document/RMO questionnaire
Future meetings, workshops, etc.
Review actions
AOB

Speaker: all

PAWG meeting: 4th Session - planning and other issues

Meeting of the PAWG

CCQM_Meeting-2024_PAWG.pptx

52 Planning of upcoming workshops

Speaker: all

53 Update of VAMAS projects

Speaker: M. Ryadnov (NPL)

54 Update of regional and NMIs’ metrology activities (ACRM,APMP......)

Speaker: all

55 Other issues, wrap up and closing of the meeting

Speaker: Claudia Swart (Physikalisch-Technische Bundesanstalt)

Transfer to the conference dinner venue: Transfer to the confernce dinner venue by public transport

Conference Dinner: Boat trip

Wednesday 9 October

Joint meeting of the bioanalysis working groups: Session 1

56 Introductions (‘tour de table’ for those present, with online participants providing name and institution in the chat box). Agree agenda

Speaker: WG Chairs

57 CAWG Update

Speaker: Julian Braybrook (NML@LGC)

10:30 Coffee Break

Joint meeting of the bioanalysis working groups: Session 2

58 NAWG Update

Speaker: Jim Huggett (NML@LGC)

59 PAWG Update

Speaker: Claudia Swart (Physikalisch-Technische Bundesanstalt)

12:30 Lunch Break

Joint meeting of the bioanalysis working groups: Session 3

60 Joint workshops

Speaker: Claudia Swart (Physikalisch-Technische Bundesanstalt)

61 WG support for RMOs, wider RMO interaction, Questionnaire

Speaker: Mojca Milavec (NIB)

62 SOGATS (WHO comparisons)

Speaker: Jim Huggett (NML@LGC)

15:00 Coffee Break

Joint meeting of the bioanalysis working groups: Session 4

63 Fire-drill update and wider pandemic TG

Speaker: Julian Braybrook (NML@LGC)

64 Task group on units for bioanalysis

Speaker: Jim Huggett (NML@LGC)

65 CCQM Task Group on Food Measurement

66 CCQM activities on Digital and FAIR Chemical and Biological Reference Data and Certificates: Challenges and Opportunities

Speaker: Robert Wielgosz (BIPM)

67 Wrap up

Speaker: WG Chairs

Thursday 10 October

Welcome: Welcome address

Convener: Prof. Dr. Tobias Schäffter (Head of PTB Berlin Institute)

CCQMPathogens_Workshop_agenda_2024.pptx

Workshop "Towards standardized pathogen measurements": Session 1

Convener: Dr Esmeralda Valiente (PTB)

CCQMPathogens_Workshop_agenda_2024.pptx

68 Quality assurance and standardization in molecular diagnostics of infections - Results from international IFCC surveys

Speaker: Prof. Dr. Parviz Ahmad-Nejad (Helios Universitätsklinikum Wuppertal)

69 Ensuring accuracy in the development and application of bioanalytical diagnostic tests for infectious disease

Speaker: Dr Jim Huggett (NML@LGC)

10:25 Coffee break

70 Antimicrobial susceptibility testing of veterinary pathogen bacteria: possibility and needs

Speaker: Dr Heike Kaspar (BVL)

Workshop "Towards standardized pathogen measurements": Session 2

Convener: Dr Megan Cleveland (NIST)

CCQMPathogens_Workshop_agenda_2024.pptx

71 Protein reference material development for increased confidence in COVID-19 testing

Speaker: Dr Bradley Stocks (NRC)

72 Advanced molecular diagnostics for the detection of high risk pathogens: exploring new technologies for scalable, rapid, on-site pathogen detection

Speaker: Dr Andreas Puyskens (RKI)

73 Boosting Accuracy in Viral Load measurements with a Powerful Duo: Isotope Dilution Mass Spectrometry and Digital Droplet PCR

Speaker: Yan Yan Beer (PTB)

74 Standardisation of Genome Amplification Techniques (SoGAT): current challenges in harmonising measurement for NAT based diagnostics

online

Speaker: Dr Neil Almond (MHRA)

12:55 Lunch break

Workshop "Towards standardized pathogen measurements": Session 3

Convener: Dr Bradley Stocks (NRC)

CCQMPathogens_Workshop_agenda_2024.pptx

75 Challenges in standardized pathogen identification and quantification in patient samples

Speaker: Prof. Dr. Udo Reischl (INSTAND)

76 The challenges in preparation of control materials for small inter-laboratory comparisons: case studies of MPXV and Listeria monocytogenes

Speaker: Dr Alexandra Bogožalec Košir (NIB)

77 Reliable quantification of control samples for the virus genome detection to assess laboratory performance in EQA schemes

Speaker: Dr Annemarie Martin (GDB)

78 Synthetic Nucleic Acids as Reference Materials for Emerging Viruses

Speaker: Dr Megan Cleveland (NIST)

Workshop "Towards standardized pathogen measurements": Final discussion

CCQMPathogens_Workshop_agenda_2024.pptx

Friday 11 October

Technical tour: Transfer from PTB Charlottenburg and Visit at BAM and Beamline in Adlershof

Convener: Rudolf Schneider

Technical_Tour_(Friday)_programme.pdf

79 Departure at PTB Berlin Charlottenburg, Bus transfer to Adlershof

Luggage can be stored at BAM

80 Visit to BAM labs: Protein Analysis, Immunoanalysis/Molecular Biology, Biophotonics, Chemical and Optical Sensing

81 Visit to BESSY II Electron Storage Ring, PTB beamline, BAM line and Metrology Light Source (PTB)

12:30 Lunch break

Lunch buffet at BAM